Kinetic Properties Of Wheat Germ Aldolase

kinetic Properties of Wheat Germ Phosphatase Results: 1. The nett ducking of sexually transmitted disease Phosphatase enzyme in this reaction was 0.07 ìM, the final inhibitor concentration was 0.67 ìM. (see sample calculation one). 2. tabular array 1. Enzyme-Substrate Reaction (Uninhibited) engender         Concentration of PNPP (mM)          volume of 5.0mM PNPP (ml)          gaudiness of dH2O (ml)         A405          hurrying*(ìmol/min) 1         0         0         4.3         0 (blank)         0 2         0.05         0.05         4.25         0.148         0.0118 3         0.075         0.075         4.225         0.199         0.0159 4         0.113         0.113         4.187         0.231         0.0184 5         0.170         0.170         4.130         0.286         0.0228 6         0.255         0.255         4.045         0.337         0.0269 7         0.384         0.384         3.916         0.398         0.0318 8         0.578         0.578         3.722         0.414         0.0330 9         0.88         0.88         3.42         0.507         0.0405 10         1.32         1.32         2.98         0.578         0.0461 11         2.0         2.0         2.3         0.655         0.0523 12         3.0         3.0         1.3         0.708         0.0565 7         0.384         0.384         3.916         0.324         0.0259 * See assay Calculation #2. 7 is a send of tube #7. 3. manipulate panel 2. Enzyme-Substrate Reaction (Inhibited) tube-shaped structure         Concentration of PNPP (mM)          mass of 5.0mM PNPP (ml)         Volume of 1.5mM K2HPO4 (ml)         Volume of dH20 (ml)         A405          focal ratio(ìmol/min) 1         0         0         1         3.3         0 (blank)         0 2         0.05         0.05         1         3.25         0.002         0.0002 3         0.075         0.075         1         3.225         0.002         0.0002 4         0.11         0.11         1         3.19         0.023         0.0018 5         0.17         0.17         1         3.13         0.070         0.0056 6         0.26         0.26         1         3.04         0.081         0.0065 7         0.38         0.38         1         2.91         0.103         0.

0082 8         0.58         0.58         1         2.72         0.116         0.0093 9         0.88         0.88         1         2.42         0.161         0.0128 10         1.32         1.32         1         1.98         0.226         0.0180 11         2.00         2.00         1         1.3         0.302         0.0241 12         3.00         3.00         1         0.3         0.418         0.0334 3. graph one 4. Graph two 5. Table 3: Km and Vmax values darn type         Km earthy         Km inhibited         Vmax unrestrained         Vmax inhibited MM*         0.28         4.00         0.72         0.95 LB         0.15         31.09         0.047         0.62 * See attached Kinetic printouts. Sample Calculations 1.         Enzyme: V1M1 = V2M2 (0.2ml)(2.5ìM Acid Phosphatase) = (7.5ml)(M2) M2 = 0.07 ìM Inhibitor: V1M1 = V2M2 (1ml)(5mM K2HPO4) = (7.5ml)(M2) M2 = 0.67 ìM 2. Velocity (aka. Enzyme Activity) Example: Tube 2 of Uninhibited a.         Use absorbance to insure concentration. c = A/(ål) = (0.148)/[(18.8x103L/mol-cm)(1cm)] = 7.87 x... If you call for to get a luxuriant essay, order it on our website: Orderessay

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